Yes, many times. Scientists can make speciation happen on small scales using artificial selection and allopolyploidy (Having two or more complete sets of chromosomes derived from different species). Scientists have created new species of plant crops, bacteria, and the famous examples of fruit flies. We can also see speciation in ring species. Ring species are distributed in more or less a straight line, such as around the rim of a valley, which forms a ring of the organism’s distribution. Each population of organism can breed with its neighbors, but they cannot breed with the organisms at the other end of the ring. Major changes take too long for us to observe directly, but we can deduce them from living organisms and from fossil remains. (Berra, Evolution and the Myth of Creationism, p. 129-130) (Dawkins, The Ancestor’s Tale, p.301-303, 307-308). Please see here and here.
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